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Reddot Biotech reddot 2 dyes
Reddot 2 Dyes, supplied by Reddot Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reddot+2/reddot+2+dyes/pm40514196-164-4-4
Average 90 stars, based on 1 article reviews
reddot 2 dyes - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Design and synthesis of novel cyclohexanecarboxamides with anticonvulsant effect by activating Nrf2-ARE pathway.
Article Snippet: This study investigated a series of novel cyclohexanecarboxamide derivatives 4a-e, 5a-e and 6a-e as anticonvulsant and neuroprotective agents.. Compounds 4a-e, 5a-e, and 6a-e were synthesized starting from cyclohexanone and aniline derivatives and evaluated for their anticonvulsant effects using maximal electroshock (MES) and subcutaneous pentylenetetrazole (scPTZ) seizure models.. The most potent compounds 4b, 5c, and 6d demonstrated 83.33 % protection in the scPTZ test, while compounds 5a and 6b showed 100 % protection in the MES test.

Article Title: Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19.
Article Snippet: The experimental setup included a gray open field measuring 60 cm by 60 cm by 26 cm and overhead cameras coupled with the computer tracking system (Noldus EthoVision XT).

Article Title: Protocatechuic acid mitigates 5-fluorouracil-triggered renal and hepatic injury in rats.
Article Snippet: Renal and hepatic contents of Nrf-2 (Cat. No: RD-NFE2L2-Ra; Reddot Biotech Inc. Co., Canada), HO-1 (Cat. No: ER1041; FineTest Co., USA), TNF-α (Cat. No: 438204; BioLegend Co., USA), IL1β (Cat. No: E0119Ra; BT LAB Co., China) and NF-κB p65 (Cat. No: E-EL-R0674; Elabscience Co., USA) were measured using ELISA technique according to the manufacturer’s instructions.

Article Title: Saroglitazar ameliorates 5- Fluorouracil-induced hepatorenal damage in rats.
Article Snippet: Rationale: Hepatotoxicity and nephrotoxicity are significant adverse effects caused in cancer patients treated with 5-Flurouracil (5-FU), a pyrimidine analogue anti-metabolite anticancer drug.. The purpose of this research was to evaluate the impact of PPAR α/γ agonist (Saroglitazar; SARO) on 5-FU-induced hepatorenal damage in rats.. Methods: Male rats were randomly assigned to four groups: control, 5-FU, 5-FU + SARO (2 mg/kg), and 5-FU + SARO (4 mg/kg).

Enzyme-linked Immunosorbent Assay:

Article Title: Dengue NS1 Antibodies Are Associated With Clearance of Viral Nonstructural Protein-1
Article Snippet: .. The Human Sialidase 2 ELISA kit (Reddot Biotech) was used according with manufacturer's instructions. ..

Incubation:

Article Title: Fibrotic response to anti-CSF-1R therapy potentiates glioblastoma recurrence.
Article Snippet: .. Samples were incubated for 5 min at RT with DAPI and Reddot, and sorted for live cells on an Astrios cell sorter (Beckman Coulter). .. Subsequently, samples were processed using the Chromium Next GEM Single Cell 30 Reagent Kits v3.1 (Dual Index, 103 Genomics).



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A. Competitive ELISA assessing the binding of His-tagged IgGs to immobilized HER2 in the presence or absence of saturating concentrations of non-His-tagged IgGs, detected with an anti-His-HRP antibody. B. Saturation binding curves of trastuzumab and pertuzumab to HER2 on live MCF7 cells. Cells were incubated with antibodies (0-400 nM) for 30 min and detected with an AF647-conjugated goat anti-human secondary antibody via flow cytometry. C. Competitive binding of of AF647-labeled trastuzumab or pertuzumab (0-300 nM) to MCF7 cells pre-saturated with 267 nM of unlabeled trastuzumab and pertuzumab, analyzed by flow cytometry. D. Binding of AF647-conjugated m66 or m75 to MCF7 cells after pre-incubation with 267 nM trastuzumab or pertuzumab. E. Binding of AF647-conjugated rabbit antibodies to MCF7 cells pre-saturated with 267 nM of trastuzumab and pertuzumab, evaluated by flow cytometry. T, trastuzumab; P, pertuzumab. Error bars, SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, T-test.

Journal: PLOS One

Article Title: Inhibition of HER2 signaling and breast cancer cell growth with a novel antibody targeting HER2 ECD III/IV

doi: 10.1371/journal.pone.0338127

Figure Lengend Snippet: A. Competitive ELISA assessing the binding of His-tagged IgGs to immobilized HER2 in the presence or absence of saturating concentrations of non-His-tagged IgGs, detected with an anti-His-HRP antibody. B. Saturation binding curves of trastuzumab and pertuzumab to HER2 on live MCF7 cells. Cells were incubated with antibodies (0-400 nM) for 30 min and detected with an AF647-conjugated goat anti-human secondary antibody via flow cytometry. C. Competitive binding of of AF647-labeled trastuzumab or pertuzumab (0-300 nM) to MCF7 cells pre-saturated with 267 nM of unlabeled trastuzumab and pertuzumab, analyzed by flow cytometry. D. Binding of AF647-conjugated m66 or m75 to MCF7 cells after pre-incubation with 267 nM trastuzumab or pertuzumab. E. Binding of AF647-conjugated rabbit antibodies to MCF7 cells pre-saturated with 267 nM of trastuzumab and pertuzumab, evaluated by flow cytometry. T, trastuzumab; P, pertuzumab. Error bars, SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, T-test.

Article Snippet: Briefly, each antibody was reacted with 20 molar equivalents of AF647 NHS ester (Biotium, 30–3007) for 2 h at room temperature protected from light.

Techniques: Competitive ELISA, Binding Assay, Incubation, Flow Cytometry, Labeling